久久夜色精品国产欧美乱-欧美日韩成人在线-国模冰莲自慰肥美胞极品人体图-来一水AV@lysav

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676 WM-266-4 人黑素瘤細(xì)胞

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

簡要描述:CRL-1676 WM-266-4 人黑素瘤細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1782

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Designations: WM-266-4

Depositors: M Herlyn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Cellular Products: proteoglycan antigen characteristic of melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 13

D7S820: 8

THO1: 7,9

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.

Age: 58 years

Gender: female

Comments: The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma.

Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-1675



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品国产精品亚洲精品| 国产精品无码久久av| 无套中出丰满人妻无码| 国产精品高潮呻吟AV久久动漫| 亚洲日韩欧美一区二区三区| 亚洲AV无码乱码在线观看 | 狠狠综合久久AV一区二区| 公交车上内裤滑进去了会怎么样| 精品国产一区二区三区AV性色| 欧美成人精品第一区二区三区| 日本熟妇色XXXXX日本免费看,| 极品少妇XXXX精品少妇偷拍| 韩国三级中文字幕HD久久精品| 一区二区视频| 国产农村妇女毛片精品久久| 野外做受又硬又粗又大视频√| 人妻含泪让粗大挺进| 乱色熟女综合一区二区三区| 成人做爰WWW免费看视频日本| 2022最新韩国理伦片在线观看| 好涨嗯太深了嗯啊用力别停| 精品人体无码一区二区三区| 性少妇freesexvideos高清| 丰满熟妇乱又伦| 天堂资源最新在线| 一炕四女被窝交换啪啪| 国产精品内射后入合集| 黑人巨大精品欧美一区二区免费| MD传媒破解版APP免费版| 又狠又深H军人高干| 亚洲精品国产电影| 欧美艳星NIKKI激情办公室| 亚洲av无码一区二区三区在线观看| 免费无遮挡无码永久在线观看视频 | JAPANESEHD熟女熟妇| 欧美性猛交XXXX免费看| 熟妇人妻av无码一区二区三区| 国产精品久久久久一区二区三区共| 清纯校花挨脔日常H| 免费人成自慰网站| 精品国产一区二区三区久久久狼 |