久久夜色精品国产欧美乱-欧美日韩成人在线-国模冰莲自慰肥美胞极品人体图-来一水AV@lysav

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>FL62891人肝細(xì)胞

人肝細(xì)胞

簡(jiǎn)要描述:CRL-11005 FL62891 人肝細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):FL62891
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1885

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-11005 FL62891 人肝細(xì)胞

Designations: FL 62891
Depositors: ImClone Systems Inc.
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen


Medium & Serum: San)ee PropagationATCC® Number: CRL-11005™ Price: $353.00


Growth Properties: adherent

Organism: Homo sapiens (hum

Morphology: fibroblast

CRL-11005 FL62891 人肝細(xì)胞

Source: Organ: liver

Cell Type: immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Age: fetus

Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 32.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 32°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999



CRL-11005 FL62891



















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
特级毛片爽WWW免费版| 亚洲AV成人精品一区二区三区| 久久亚洲精品中文字幕| 被黑人伦流澡到高潮HNP动漫| 696969大但人文艺术正道| 人妻丰满熟妇AV无码区HD| 公交车强摁做开腿呻吟| 久久久久99精品成人片| 亚洲AV永久无码精品三区在线 | 亚洲亚洲精品AV在线动态图| 丰满少妇被猛烈高清播放| 我强行与岳的性关系| 99久久国产综合精品麻豆| 麻花传媒剧国产MV网站| SAO货撅起你的贱屁股来| 日韩乱码人妻无码系列中文字幕| 十九岁日本电影免费完整版观看| 色一情一乱一乱一区99AV| 最近中文字幕国语免费高清6| 精品久久人妻AV中文字幕| 校草喝下春药被男生玩弄| 无码少妇高潮浪潮AV久久| you jizz jizz japan日本| 69精品丰满人妻无码视频A片| 真人性做爰直播| 24小时日本在线WWW免费的| 男男开小嫩苞好深啊H窑子开| 成人无遮挡18禁免费视频| 无码人妻精品一区二区三区66 | 久久精品国产亚洲AV成人| 最近高清中文在线字幕在线观看| 国产成人一区二区三区影| 免费A级毛片黄A片高清在线播放| 人人爽人人爽人人爽| 国产农村熟妇出轨VIDEOS| 国产精品毛片无遮挡高清| 成人性生交大片免费看中文| 亚洲精品一区久久久久久| 巨大黑人极品VIDEOS精品| 一女大战七个黑人到喷浆| 东北浓毛老妇国语对白|