久久夜色精品国产欧美乱-欧美日韩成人在线-国模冰莲自慰肥美胞极品人体图-来一水AV@lysav

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>Panc 04.03細胞, 人胰腺癌細胞

Panc 04.03細胞, 人胰腺癌細胞

簡要描述:Panc 04.03細胞, 人胰腺癌細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1776

產(chǎn)品分類

Product Category

詳細介紹

Panc 04.03細胞, 人胰腺癌細胞

Panc 04.03 (ATCC® CRL-2555™)

Organism  Homo sapiens, human

Tissue  pancreas

Product Format  frozen

Morphology  epithelial

Culture Properties  adherent

Biosafety Level  1

Disease  adenocarcinoma

Age  70 years adult

Gender  male

Ethnicity  White

Storage Conditions  liquid nitrogen vapor phase

Derivation  Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Clinical Data  70 years

Caucasian

male

Antigen Expression  MHC class I +; MHC class II -

RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Blood type O; Rh+

Oncogene  K-ras + RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Genes Expressed  cytokeratins 7 and 18 RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Tumorigenic  Yes

Effects  Yes, forms tumors in nude or SCID mice

Comments  The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

The cells have a reported plating efficiency of 80%. Ref

Panc 04.03細胞, 人胰腺癌細胞

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

•20 Units/ml human recombinant insulin

•fetal bovine serum to a final concentration of 15%


Subculturing  Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: 1:2 to 1:3

Medium Renewal: Add media once per week. Fluid change one to two times per week.


Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation  Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions  Temperature: 37°C




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
免费国产裸体美女视频全黄| 久久夜色精品国产| 亚洲第一AV网站| 上司人妻互换中文字幕| 精品一区二区三区免费毛片爱| 国产重口老太和小伙A片| 秋霞成人午夜鲁丝一区二区三区| 国产欧美熟妇另类久久久| 国产成人无码精品久久久免费| 特级AAAAAAAAA毛片免费视频| 永久免费AV无码网站性色AV| 国产精品欧美一区二区三区不卡| 日本丰满熟妇BBXBBXHD| 亚洲AV中文无码乱人伦在线观看| 无码日本精品XXXXXXXXX| 老熟妇高潮一区二区三区无码久久精品国产亚洲AV影片 | 天堂А√在线中文在线| 亚洲色大成网站WWW| 亚洲,欧美,日韩,国产| 人人摸人人操| 天天操,天天干,天天日| 午夜亚洲福利在线老司机| 欧美亚洲一区二区三区| 啊灬啊灬啊灬快灬高潮了女| 国产成人无码AV| 色综合久久久无码中文字幕| 国产精品高潮呻吟AV久久动漫| 亚洲欧美中文日韩在线V日本| 欧美日韩在线视频| 亚洲色大成网站WWW| 日韩精品久久久久久免费| 大明荫蒂女人毛茸茸| 亚洲AV综合AV一区二区三区| 日韩三级| 久久久久99精品成人片三人毛片| AV无码精品一区二区三区| 日韩人妻精品无码一区二区三区 | 国产又爽又猛又粗的视频A片| 精品AV无码国产一区二区| 久久人人爽人人爽人人片AV高请| 亚洲AV无码码潮喷在线观看|